Producto del catálogo Hycult Biotech. Referencia del fabricante: HK3051.
The C3NeF detection ELISA kit is intended as a prescreening tool for the in vitro qualitative detection of C3NeF autoantibodies in heat-inactivated serum or plasma. C3NeF is an IgG autoantibody that binds to the Bb portion of the C3 convertase C3bBb(P), thereby stabilizing the complex and prolonging its half-life, resulting in sustained activation of the complement alternative pathway (AP). Sustained AP activation can contribute to disease, and the presence of C3NeF has been reported in several complement-mediated disorders, most prominently in C3 glomerulopathy, including dense deposit disease and C3 glomerulonephritis, but also in atypical hemolytic uremic syndrome and related conditions characterized by dysregulated alternative pathway activation.
As a prescreening tool, the C3NeF detection ELISA can support the identification of patients who may have C3NeF autoantibodies and may benefit from further clinical evaluation and confirmatory testing.
The assay consists of a C3b-coated plate to which functional Factor B (FB) and Factor D (FD) are added to allow formation of the C3bBb convertase, to which potentially present C3NeFs can bind. Bound C3NeFs are detected using a peroxidase-conjugated anti-human IgG antibody, which catalyzes the conversion of the TMB substrate into a measurable color signal.
- Reactividad / especie: Human
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